d9542 antifade mounting medium vector laboratories Search Results


d9542  (Vector Laboratories)
97
Vector Laboratories d9542
D9542, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl substrate  (Thermo Fisher)
90
Thermo Fisher ecl substrate
Ecl Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4′,6-diamidino-2-phenylindole dapi  (Merck KGaA)
90
Merck KGaA 4′,6-diamidino-2-phenylindole dapi
4′,6 Diamidino 2 Phenylindole Dapi, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield antifade mounting medium with dapi  (Vector Laboratories)
98
Vector Laboratories vectashield antifade mounting medium with dapi
(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with <t>DAPI,</t> Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield antifade media  (Fisher Scientific)
90
Fisher Scientific vectashield antifade media
(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with <t>DAPI,</t> Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.
Vectashield Antifade Media, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4′,6-diamidino-2-phenylindole dapi #d9542  (Merck KGaA)
90
Merck KGaA 4′,6-diamidino-2-phenylindole dapi #d9542
(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with <t>DAPI,</t> Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.
4′,6 Diamidino 2 Phenylindole Dapi #D9542, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4′,6-diamidino-2-phenylindole dapi #d9542/product/Merck KGaA
Average 90 stars, based on 1 article reviews
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dapi (4,6-diamidino-2-phenylindole)  (Millipore)
90
Millipore dapi (4,6-diamidino-2-phenylindole)
(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with <t>DAPI,</t> Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.
Dapi (4,6 Diamidino 2 Phenylindole), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector laboratory pi 2000 anti mouse  (Vector Laboratories)
96
Vector Laboratories vector laboratory pi 2000 anti mouse
(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with <t>DAPI,</t> Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.
Vector Laboratory Pi 2000 Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reduced glutation  (Millipore)
90
Millipore reduced glutation
(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with <t>DAPI,</t> Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.
Reduced Glutation, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with DAPI, Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.

Journal: bioRxiv

Article Title: cGAS-mediated IFN-I signaling contributes to disease progression in drug-refractory epilepsy

doi: 10.64898/2026.01.30.702860

Figure Lengend Snippet: (a) Schematic showing the experiment design of the EV isolation and ICH in Dravet mice. (b) Confocal image of the brain section stained with DAPI, Iba1, and double-strain DNA (dsDNA), displaying sampling windows in hippocampus CA1. Bar = 1000 ⎧m. (c) 100x images from Scn1a +/+ and Scn1a +/- mouse showing immuno-fluorescence signals of DAPI, Iba1, and dsDNA in hippocampus CA1 single microglia cells. Bar=10 ⎧m. (d) Analysis of total dsDNA puncta in the cytoplasma of single hippocampal microglia cells and comparison between the two groups. N=79 cells ( Scn1a +/+ ), 101 cells ( Scn1a +/- ). N = 4 mice per group. Mixed-effects model ANOVA (F(1,6)= 9.742). (e) NanoFCM showed significant changes in size-based distribution in of hippocampal tissue EVs. N = 4 mice per group, two-way ANOVA (F (2, 18) = 78.95), Sidak’s multiple comparisons test. (f) Experimental scheme for the in vitro identification of the endogenous neuronal ligand for cGAS activation in primary microglia. (g) Acute PTZ-induced spontaneous epileptic activity in primary neurons. Up: representative fluorescence images of neurons expressing GCaMP8f (up). Scale bar: 50 μm. Down: calcium times course of each cell from above image showed spontaneous epileptic events after treated with 10 mM PTZ. (h) Representative western blot image for pTBK1, TBK1, and β-actin in primary Cgas+/+ and Cgas-/- microglia treated with untreated neuronal CM, HT-DNA, and PTZ-treated neuronal CM. (i) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Cgas+/+ : untreated CM, n=6, PTZ-treated CM, n=6. Cgas-/- : untreated CM, n=4, PTZ-treated CM, n=5. Two-way ANOVA with uncorrected Fisher’s LSD. (j) Experimental scheme for the in vitro identification of the endogenous neuronal ligand in PTZ-treated primary microglia (up). Representative western blot image for pTBK1, TBK1, and β-actin in primary wt microglia treated with control (ctrl) and PTZ (bottom). (k) Quantification of the ratio of pTBK1 to total TBK1 protein level. Each dot represents one well from two biologically independent experiments. Ctrl , n=7, PTZ, n=9. Welch’s t test.

Article Snippet: The following day, sections were washed thoroughly and incubated in appropriate secondary antibodies (1:500; Invitrogen) for 1 h. Sections were washed, mounted on slides using Vectashield antifade mounting medium with DAPI (Vector Laboratories, H-1200; or Sigma, D9542), and imaged.

Techniques: Isolation, Staining, Sampling, Fluorescence, Comparison, In Vitro, Activation Assay, Activity Assay, Expressing, Western Blot, Control